SIMPLE: software for ab inito reconstruction of flexible single-particles
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چکیده
We have developed an ab initio single-particle 3D reconstruction algorithm for cryo-EM that does not rely on contrast enhancement by negative staining, assumptions about particle symmetry, or knowledge about the character of the heterogeneity [1-3]. Here we announce the release of the methods as an open source program package SIMPLE (Single-particle IMage Processing Linux Engine). SIMPLE enables semi-automated reconstruction of all structural states in the single-particle ensemble. SIMPLE is easy to use, yet provides a low-level image-processing engine with all the generated data, Euler angles, state assignments etc. readily accessible in text files. Documentation and executable binaries for Linux and Mac OSX are available at http://simple.stanford.edu.
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تاریخ انتشار 2012